# [Salmonellosis in Poultry](/knowledge/bacteria/avian-bacteria/salmonella-in-poultry-veterinary-reference): Public Health and Flock Management ## Key Takeaways - *Salmonella enterica* subsp. *enterica* is the causative agent, with over 2,500 serovars; host-adapted serovars (*S. Gallinarum*, *S. Pullorum*) cause severe systemic disease, while non-typhoidal serovars (*S. Enteritidis*, *S. Typhimurium*, *S. Infantis*) are significant foodborne pathogens often causing subclinical infections in poultry. - Transmission occurs vertically (via egg contamination) and horizontally through fecal-oral routes, with critical control points including hatcheries and contaminated feed, water, or equipment; wildlife and insects can also act as vectors. - Pathogenesis involves oral ingestion, survival in the gastrointestinal tract, invasion of host cells via type III secretion systems (T3SS), and modulation of host immune responses, particularly within macrophages. - Clinical signs range from acute systemic disease (anorexia, depression, diarrhea, high mortality) in young birds infected with host-adapted serovars to often subclinical infections in adults with non-typhoidal serovars, posing a significant food safety risk. - Diagnosis relies on bacteriological culture (gold standard), serological assays (ELISA for flock screening), and rapid molecular methods (PCR, qPCR, WGS for high-resolution typing and AMR profiling). - Control strategies integrate stringent biosecurity, vaccination (live and killed vaccines), targeted feed/water additives (organic acids, probiotics), environmental monitoring, and the judicious use of antimicrobials due to widespread antimicrobial resistance, with emerging alternatives like phage therapy and plant extracts showing promise. --- ## Etiology and Serovar Diversity [Salmonellosis in poultry](/knowledge/bacteria/avian-bacteria/salmonella-in-poultry-veterinary-reference) is caused by infection with bacteria of the genus *Salmonella* within the family Enterobacteriaceae. The primary etiological agent is *Salmonella enterica* subsp. *enterica*, which encompasses over 2,500 serovars distinguished by somatic (O) and flagellar (H) antigens [1, 2]. In poultry, the most clinically and epidemiologically relevant serovars are broadly categorized into two groups: host-adapted serovars and non-typhoidal, broad-host-range serovars. Host-adapted serovars include *Salmonella* Gallinarum and *Salmonella* Pullorum, which cause fowl typhoid and pullorum disease, respectively [2]. These serovars are highly pathogenic to chickens and turkeys, causing systemic, often fatal infections, particularly in young birds [2]. Non-typhoidal serovars such as *Salmonella* Enteritidis, *Salmonella* Typhimurium, *Salmonella* Infantis, *Salmonella* Heidelberg, and *Salmonella* Kentucky are of major concern due to their ability to colonize the avian gastrointestinal tract without causing clinical disease in many cases, yet they represent a significant reservoir for human foodborne illness [3, 4, 5, 6]. The serovar distribution varies geographically and over time, influenced by poultry production systems, biosecurity practices, and international trade [3, 7, 8]. For instance, *Salmonella* Infantis has emerged as a dominant serovar in many regions, often carrying the pESI megaplasmid which harbors multiple antimicrobial resistance genes [6]. Similarly, *Salmonella* Kentucky ST198 has demonstrated clonal spread in poultry market environments, indicating a persistent environmental reservoir. ## Epidemiology and Transmission Dynamics The epidemiology of [salmonellosis in poultry](/knowledge/bacteria/avian-bacteria/salmonellosis-in-poultry-clinical-presentation-public-health-implications-and-control-strategies) is complex and multifactorial. Transmission occurs through both vertical and horizontal routes. Vertical transmission is particularly important for *Salmonella* Enteritidis, which can colonize the reproductive tract of laying hens and contaminate the internal contents of eggs before shell formation [5, 9]. Horizontal transmission occurs via the fecal-oral route, through contaminated feed, water, litter, equipment, and personnel [5, 10]. Hatchery environments are critical control points, as *Salmonella* can be isolated from dead-in-shell eggs and hatchery surfaces, leading to early colonization of chicks [1]. Once introduced into a flock, *Salmonella* can spread rapidly. The bacterium persists in the poultry house environment, surviving in dust, feces, and litter for extended periods. *Salmonella* Minnesota, for example, has been shown to persist in poultry slaughterhouse environments, with genotypic characterization revealing clonal strains that survive cleaning and disinfection protocols. The role of wildlife and insects as mechanical vectors should not be underestimated, as rodents, flies, and wild birds can introduce and maintain *Salmonella* within a poultry operation [10]. The population dynamics of *Salmonella* within a flock are influenced by host factors such as age, immune status, and gut microbiota composition, as well as pathogen factors including virulence gene repertoire and antimicrobial resistance profile [11, 12, 13, 14]. ## Pathogenesis and Host-Pathogen Interactions The pathogenesis of *Salmonella* infection in poultry begins with oral ingestion of the bacterium. After surviving the acidic environment of the proventriculus and gizzard, *Salmonella* reaches the intestine, where it must compete with the resident microbiota and overcome mucosal barriers [10, 11]. The bacterium uses type III secretion systems (T3SS) encoded by pathogenicity islands to inject effector proteins into host cells, facilitating invasion of intestinal epithelial cells and M cells overlying Peyer's patches [10, 15]. Following invasion, *Salmonella* can be phagocytosed by macrophages and dendritic cells, surviving and replicating within these cells by modulating host immune responses [11, 16, 17]. Single-cell transcriptomic profiling has revealed that *Salmonella* Enteritidis infection in chickens induces an expansion of innate-like cytotoxic intraepithelial lymphocytes, which play a role in early immune defense [11]. The host immune response is tightly regulated; for instance, SIRT1 has been shown to negatively regulate immune responses, thereby attenuating host resistance to *Salmonella* infection [17]. Macrophage cell lines, such as the HD11 chicken macrophage-like cell line, are used to study intracellular survival mechanisms. Organic acids have been shown to impede *Salmonella* infection of HD11 cells by modulating itaconate gene expression, a metabolite with antimicrobial properties [16]. The bacterium also employs strategies to subvert host defenses, including the use of T3SS-1 for initial invasion and T3SS-2 for intracellular survival and systemic spread [15]. ## Clinical Signs in Poultry Clinical manifestations of [salmonellosis in poultry](/knowledge/bacteria/avian-bacteria/salmonellosis-in-poultry-clinical-presentation-public-health-implications-and-control-strategies) vary widely depending on the serovar, the age and immune status of the bird, and the infectious dose. Infections with host-adapted serovars *Salmonella* Gallinarum and *Salmonella* Pullorum typically cause acute systemic disease. In young chicks and poults, pullorum disease is characterized by anorexia, depression, white pasty diarrhea (sometimes adhering to the vent, a condition known as "pasting"), weakness, and high mortality [2]. Fowl typhoid, caused by *Salmonella* Gallinarum, affects older birds and presents with similar signs, including septicemia, liver necrosis, and splenomegaly [2]. In contrast, infections with non-typhoidal serovars such as *Salmonella* Enteritidis and *Salmonella* Typhimurium are often subclinical in adult birds, particularly in well-adapted commercial lines [5, 9]. However, in young birds, these serovars can cause diarrhea, dehydration, and mortality. Even in the absence of clinical signs, infected birds shed large numbers of bacteria in their feces, contaminating the environment and carcasses at slaughter [3, 5]. This subclinical carrier state is a major challenge for flock management and [food safety](/knowledge/bacteria/livestock-bacteria/cooking-chicken-bacteria-prevention). The term "chicken breast salmonella meme" has emerged in popular culture to describe the public's awareness of the risk of *Salmonella* contamination in raw poultry products, particularly chicken breast meat, highlighting the disconnect between the asymptomatic infection in the bird and the potential for human illness. ## [Food Safety](/knowledge/bacteria/livestock-bacteria/cooking-chicken-bacteria-prevention) Implications and Public Health Salmonellosis is one of the most important foodborne zoonoses worldwide, and poultry products (meat and eggs) are among the primary vehicles for human infection [3, 5, 6, 18]. Human infection typically results from the consumption of undercooked eggs or meat, or through cross-contamination in the kitchen. The public health burden is substantial, with millions of cases of salmonellosis reported annually, leading to significant morbidity, hospitalization, and occasional mortality [18]. The [food safety](/knowledge/bacteria/livestock-bacteria/cooking-chicken-bacteria-prevention) risk is compounded by the emergence of multidrug-resistant (MDR) and extensively drug-resistant (XDR) *Salmonella* strains in poultry populations [1, 2, 8, 12, 19]. Antimicrobial resistance (AMR) genes, including those encoding resistance to cephalosporins (e.g., *bla* genes) and fluoroquinolones, are frequently identified in *Salmonella* isolates from poultry [8, 19]. The presence of class 1 integrons, which can capture and express gene cassettes conferring resistance to multiple antibiotic classes, is a particular concern in XDR strains [1]. The pESI megaplasmid, commonly found in *Salmonella* Infantis, carries a suite of resistance genes and has been identified as a major driver of AMR dissemination in poultry [6]. Quantile regression forest models using multi-source [food safety](/knowledge/bacteria/livestock-bacteria/cooking-chicken-bacteria-prevention) surveillance data have been developed to provide early warning of *Salmonella* foodborne risk, integrating data from farm to fork [18]. ## Diagnostics Accurate and timely diagnosis of [salmonellosis in poultry](/knowledge/bacteria/avian-bacteria/salmonellosis-in-poultry-clinical-signs-diagnosis-and-control) is essential for effective flock management and public health surveillance. Diagnostic methods can be broadly classified into bacteriological culture, serological assays, and molecular techniques. Bacteriological culture remains the gold standard for *Salmonella* isolation. Samples (e.g., cloacal swabs, fecal samples, organ tissues, environmental swabs, or feed) are pre-enriched in buffered peptone water, followed by selective enrichment in media such as Rappaport-Vassiliadis broth or tetrathionate broth, and then plated onto selective agar media (e.g., XLD agar, brilliant green agar, or [MacConkey agar](/knowledge/diagnostics/microbiology/macconkey-agar-selective-differential-enteric)) [3, 7, 12]. Suspect colonies are confirmed by biochemical tests and serotyping using O and H antisera [3, 7]. Serological methods are widely used for flock-level screening. Enzyme-linked immunosorbent assays (ELISAs) can detect antibodies against *Salmonella* in serum or egg yolk. An indirect ELISA based on the Sptp protein has been established for detecting *Salmonella* infection in poultry, offering high sensitivity and specificity [20]. These assays are useful for monitoring vaccination responses and identifying infected flocks. Molecular diagnostics, particularly polymerase chain reaction (PCR) and real-time PCR, offer rapid and sensitive detection of *Salmonella* DNA directly from samples. Conventional PCR targeting the *invA* gene is a standard method for genus-level identification [1, 3]. For serovar-level discrimination, multiplex PCR assays targeting serovar-specific genes are employed. Advanced molecular typing methods, including core-genome multilocus sequence typing (cgMLST) and whole-genome sequencing (WGS), provide high-resolution epidemiological data for tracking transmission chains and identifying AMR and virulence genes [4, 6, 8, 19]. WGS is increasingly used in surveillance programs to characterize the genetic determinants of virulence and resistance [1, 4, 19]. The following table summarizes the key diagnostic modalities: | Diagnostic Method | Principle | Application | Advantages | Limitations | |----------|------|-------|------|-------| | Bacteriological Culture | Selective enrichment and isolation on agar | Gold standard for isolation and serotyping | Provides live isolate for further characterization | Time-consuming (3-5 days); requires skilled personnel | | Serology (ELISA) | Detection of anti-Salmonella antibodies | Flock-level screening; vaccination monitoring | Rapid; high throughput; non-invasive (egg yolk) | Cannot distinguish active infection from past exposure; serovar cross-reactivity | | Conventional PCR | Amplification of *Salmonella*-specific genes (e.g., *invA*) | Rapid detection from samples | High sensitivity and specificity; rapid (hours) | Requires specialized equipment; may not differentiate viable from non-viable cells | | Real-Time PCR (qPCR) | Quantification of target DNA | Quantification of bacterial load | Quantitative; reduced contamination risk | Higher cost per sample | | Whole-Genome Sequencing (WGS) | High-throughput sequencing of entire genome | Epidemiological typing; AMR and virulence gene profiling | Maximum resolution; comprehensive data | High cost; requires bioinformatics expertise | ## Treatment and Antimicrobial Resistance The treatment of clinical [salmonellosis in poultry](/knowledge/bacteria/avian-bacteria/salmonellosis-in-poultry-clinical-signs-diagnosis-and-control) is complicated by the widespread emergence of antimicrobial resistance. In many countries, the use of antibiotics in food-producing animals is strictly regulated to minimize the selection and spread of resistance. For systemic infections caused by host-adapted serovars, treatment with antibiotics such as fluoroquinolones (e.g., enrofloxacin) or third-generation cephalosporins (e.g., ceftiofur) may be considered, but resistance to these agents is increasingly reported [1, 2, 8, 19]. Phenotypic and molecular characterization of *Salmonella* isolates from poultry consistently reveals high rates of resistance to tetracyclines, sulfonamides, and aminopenicillins [2, 3, 7, 12]. Given the limitations of antibiotic therapy, alternative strategies for controlling *Salmonella* in poultry have been extensively investigated. These include the use of organic acids, prebiotics, probiotics, synbiotics, bacteriophages, and plant-derived compounds [13, 14, 15, 16, 21, 22, 23, 24, 25, 26]. Organic acids, such as butyric acid and formic acid, can reduce *Salmonella* colonization by lowering the pH of the gastrointestinal tract and modulating host immune responses, including itaconate gene expression [16]. Dietary supplementation with *Bacillus subtilis* has been shown to reduce *Salmonella* Pullorum infection in broilers, likely through competitive exclusion and modulation of the gut microbiota [13]. Synbiotics, combinations of probiotics and prebiotics, have also demonstrated efficacy in controlling *Salmonella* infection in broilers [14]. Bacteriophage therapy represents a promising approach for targeting MDR *Salmonella*. Lytic phages specific to *Salmonella* serovars, including *Salmonella* Gallinarum and *Salmonella* Enteritidis, have been isolated and characterized for their ability to lyse bacterial cells and disrupt biofilms [24, 25, 26]. Phage cocktails targeting multiple receptors have been shown to reduce *Salmonella* Enteritidis colonization in chicks and modulate the cecal microbiome. Similarly, cationic liposome-fused endolysins (e.g., Lys40) can overcome the outer membrane barrier of Gram-negative bacteria and enhance survival in *Salmonella*-infected chicks [22]. Plant-derived compounds, such as bamboo polyphenols, oregano essential oil, and *Houttuynia cordata* extract, have demonstrated anti-Salmonella activity in vitro and in vivo [15, 21, 23]. Bamboo polyphenols protect against *Salmonella* Enteritidis in chickens by modulating inflammation, maintaining intestinal barrier integrity, and altering the gut microbiota [21]. Oregano essential oil has been evaluated as a pre-harvest tool to reduce *Salmonella* Enteritidis in market-age broilers [23]. *Houttuynia cordata* extract targets T3SS-1, thereby inhibiting *Salmonella* invasion of host cells [15]. Fermented shallot bulb, using *Lactiplantibacillus plantarum*, has also shown potential as an antibiotic alternative against *Salmonella* Pullorum infection in broilers. Systems pharmacology-based studies, such as those investigating Wengxian granules, have elucidated the multi-target mechanisms of traditional Chinese medicine formulations against [avian salmonellosis](/knowledge/bacteria/avian-bacteria/avian-salmonellosis-chicken-salmonella-comprehensive-guide). ## Control and Flock Management Effective control of [salmonellosis in poultry](/knowledge/bacteria/avian-bacteria/salmonellosis-poultry-farm-to-table) requires a comprehensive, multi-faceted approach encompassing biosecurity, vaccination, feed and water management, and environmental monitoring. The following Mermaid diagram illustrates a decision tree for integrated *Salmonella* control in a broiler flock: ```mermaid flowchart TD A["Start: Flock Management Plan"] --> B{"Biosecurity Measures in Place?"} B -->|"No"| C["Implement Biosecurity Protocols"] B -->|"Yes"| D{"Feed and Water Additives?"} C --> D D -->|"No"| E["Consider Organic Acids, Probiotics, Synbiotics"] D -->|"Yes"| F{"Vaccination Program?"} E --> F F -->|"No"| G["Evaluate Live or Killed Vaccines"] F -->|"Yes"| H{"Routine Monitoring?"} G --> H H -->|"No"| I["Implement Culture and PCR Surveillance"] H -->|"Yes"| J{"Positive for Salmonella?"} I --> J J -->|"No"| K["Continue Current Management"] J -->|"Yes"| L["Identify Serovar and AMR Profile"] L --> M["Implement Targeted Interventions: Phage Therapy, Plant Extracts, Enhanced Hygiene"] M --> N["Re-test and Verify Clearance"] N --> K ``` Biosecurity is the cornerstone of *Salmonella* control. This includes strict protocols for personnel hygiene, dedicated footwear and clothing for each house, pest control (rodents, flies, and litter beetles), and cleaning and disinfection of facilities between flocks [5, 10]. All-in/all-out production systems reduce the risk of carryover infection between flocks. Hatchery hygiene is critical, as *Salmonella* can be introduced via contaminated eggs or hatchery equipment [1]. Vaccination is a key tool for reducing *Salmonella* carriage and shedding in poultry. Both live attenuated and killed (bacterin) vaccines are available. Live vaccines, often based on attenuated *Salmonella* Enteritidis or *Salmonella* Typhimurium strains, can be administered orally via drinking water or spray, inducing mucosal and systemic immunity [9, 27, 28, 29, 30]. Vaccination programs using two different live *Salmonella* vaccines have been evaluated for efficacy against *Salmonella* Enteritidis, *Salmonella* Typhimurium, and *Salmonella* Gallinarum in brown layer hens, demonstrating variable protection depending on the challenge serovar [9]. Recombinant attenuated *Salmonella* Enteritidis vectors have been developed to deliver heterologous antigens, such as *Clostridium perfringens* toxins, for dual protection against salmonellosis and [necrotic enteritis](/knowledge/bacteria/avian-bacteria/necrotic-enteritis-poultry) [27, 29]. Novel immunogenic antigens for *Salmonella* Enteritidis vaccines continue to be identified and characterized for protective efficacy [28]. Outer membrane vesicle (OMV) overproducing mutants of *Salmonella* Enteritidis are also being explored as vaccine candidates [30]. Feed and water management strategies include the use of organic acids, probiotics, prebiotics, and synbiotics to create a hostile environment for *Salmonella* in the gastrointestinal tract and to promote a competitive gut microbiota [13, 14, 16]. The use of bacteriophages and plant extracts as feed additives is an area of active research [15, 21, 23, 24, 25, 26]. Environmental monitoring through routine sampling of litter, dust, and water is essential for early detection of *Salmonella* introduction. Molecular typing of isolates, including cgMLST and WGS, allows for the tracking of strains within and between farms, facilitating targeted interventions [4, 6, 8]. The emergence of MDR and XDR strains, particularly those carrying mobile genetic elements like integrons and megaplasmids, underscores the need for continuous surveillance and the prudent use of antimicrobials [1, 2, 6, 8, 12, 19]. ## Related Clinical & Scientific Guides * [Duck Diseases: A Comprehensive Overview for Veterinary Practitioners](/knowledge/bacteria/general/duck-diseases-comprehensive-overview-veterinary) * [Salmonella Dublin in Cattle: Emerging Pathogen, Diagnostic Challenges, and Public Health Impact](/knowledge/bacteria/general/salmonella-dublin-cattle-emerging-pathogen-diagnostic-public-health) * [Mycoplasma Infections in Poultry: Vaccination Strategies and Control Programs](/knowledge/bacteria/general/mycoplasma-infections-in-poultry-vaccination-strategies-and-control-programs) ## References [1] Eidaroos NH, Khafagy AR, Eldein AE, et al. Virulence and Antimicrobial Resistance Gene Profiling of Salmonella Isolated from Dead-in-Shell Eggs and Hatchery Environments with Emphasis on Class 1 Integron Gene Cassette Sequencing in XDR Strains. Microb Pathog. 2026. URL: https://pubmed.ncbi.nlm.nih.gov/42331072/ [2] Kingshuk MMR, Alam SB, Rahman MS, et al. The Landscape of Salmonella enterica Serovar Gallinarum-Pullorum Antimicrobial Resistance in Bangladesh's Poultry Industry: A Combined Phenotypic and Molecular Study. Microbiologyopen. 2026. URL: https://pubmed.ncbi.nlm.nih.gov/42271175/ [3] Pelyuntha W, Yamik DY, Khongkhai H, et al. Serovar Distribution, Virulence Gene Profiles, and Antimicrobial Resistance of Salmonella Isolated from Chicken Meat from the Broiler Slaughterhouse and Processing Plant in Central Thailand. Foodborne Pathog Dis. 2026. URL: https://pubmed.ncbi.nlm.nih.gov/42184212/ [4] Hetman B, Pearl D, Reid-Smith R, et al. Core-genome multilocus sequence types of the IncI1 plasmids identified in Salmonella enterica subsp. Enterica serovars Heidelberg and Kentucky from Canadian poultry production are associated with factors related to production, year, and geographical region. Can J Microbiol. 2026. URL: https://pubmed.ncbi.nlm.nih.gov/42091221/ [5] Ayala Velastegui D, Shariat NW. Investigating the differences in Salmonella serovar transmission within broiler production. Front Vet Sci. 2026. URL: https://pubmed.ncbi.nlm.nih.gov/42078842/ [6] Poudel S, Wang J, Bourassa D, et al. Population dynamics and genomic characterization of Salmonella Infantis reveal poultry as a major reservoir of antimicrobial resistance genes and pESI megaplasmid. Microbiol Spectr. 2026. URL: https://pubmed.ncbi.nlm.nih.gov/42023865/ [7] Cartelo LA, Salhi O, Boumahdi Merad Z, et al. Occurrence, antimicrobial resistance and molecular characterization of Salmonella spp. from broiler chickens in Northern Algeria. Braz J Microbiol. 2026. URL: https://pubmed.ncbi.nlm.nih.gov/42149349/ [8] Khan MAS, Rafi MA, Hossen MA, et al. Clonal spread of multidrug-resistant Salmonella Kentucky ST198 in poultry market [9] Filho RCP, Sarabia J, Sesti L, et al. Evaluation of the Efficacy of Different Vaccination Programs Using Two Live Salmonella Vaccines Against Salmonella Enteritidis, Salmonella Typhimurium, and [Salmonella Gallinarum](/knowledge/bacteria/avian-bacteria/salmonella-gallinarum-pullorum-fowl-typhoid-pullorum-disease) in Brown Layer Hens. Avian Dis. 2026. URL: https://pubmed.ncbi.nlm.nih.gov/41973010/ [10] Thiers I, Lories B, Steenackers H. In vivo models to study gastrointestinal Salmonella infections. Gut Microbes. 2026. URL: https://pubmed.ncbi.nlm.nih.gov/41986923/ [11] Majeed S, Shah BR, Aryal B, et al. Single-cell transcriptomic profiling reveals innate-like cytotoxic intraepithelial lymphocyte expansion during Salmonella Enteritidis infection in chickens. Front Immunol. 2026. URL: https://pubmed.ncbi.nlm.nih.gov/42317345/ [12] Nath S, Habib M, Banerjee J, et al. Understanding antimicrobial resistance dynamics of non-typhoidal [Salmonella in chickens](/knowledge/bacteria/avian-bacteria/salmonella-chickens-clinical-signs-zoonotic-diagnosis) and ducks - a prospective study from West Bengal, India. Braz J Microbiol. 2026. URL: https://pubmed.ncbi.nlm.nih.gov/42113384/ [13] Chen Y, Li H, Zhang X, et al. Dietary Bacillus subtilis Group Reduces the General Infection of Salmonella Pullorum in Broiler Chicken. Antibiotics (Basel). 2026. URL: https://pubmed.ncbi.nlm.nih.gov/42041352/ [14] Salim AA, Mohamed NA, El-Gammal GA, et al. The role of synbiotic in controlling Salmonella infection in broilers. Sci Rep. 2026. URL: https://pubmed.ncbi.nlm.nih.gov/41998070/ [15] Wang T, Cai S, Zhang J, et al. Houttuynia cordata extract protects against Salmonella infection by targeting type III secretion system 1. Poult Sci. 2026. URL: https://pubmed.ncbi.nlm.nih.gov/42025005/ [16] Marcu D, Balta I, Gundogdu O, et al. Organic acids impede Salmonella infection of chicken macrophage-like cell line (HD11) by modulating itaconate gene expression. Avian Pathol. 2026. URL: https://pubmed.ncbi.nlm.nih.gov/42159720/ [17] Liu S, Gao Q, Zhang J, et al. SIRT1 Attenuates Host Resistance to Salmonella Infection by Negatively Regulating the Immune Responses. FASEB J. 2026. URL: https://pubmed.ncbi.nlm.nih.gov/42033180/ [18] Liu W, Zhou Y, Yan R, et al. Quantile regression forest-based early warning of Salmonella foodborne risk using multi-source [food safety](/knowledge/bacteria/livestock-bacteria/cooking-chicken-bacteria-prevention) surveillance data. Food Res Int. 2026. URL: https://pubmed.ncbi.nlm.nih.gov/41942211/ [19] Yang Q, Zhang J, Chen T, et al. Global prevalence and distribution of bla-harboring Salmonella: A genome-wide association study of cephalosporin resistance mechanisms. Food Microbiol. 2026. URL: https://pubmed.ncbi.nlm.nih.gov/41963065/ [20] Xie L, Xia Y, Shen R, et al. Establishment of an indirect ELISA method for detecting Salmonella infection based on Sptp protein in poultry. J Microbiol Methods. 2026. URL: https://pubmed.ncbi.nlm.nih.gov/42219045/ [21] Liao Q, Zheng L, Huang J, et al. Bamboo Polyphenols Protect Against Salmonella Enteritidis in Chickens by Modulating Inflammation, Barrier Integrity, and Microbiota. Animals (Basel). 2026. URL: https://pubmed.ncbi.nlm.nih.gov/42121711/ [22] Huo Z, Li Y, Zhong C, et al. Cationic Liposome-Fused Endolysin Lys40 Overcomes Outer Membrane Barriers and Enhances Survival in Salmonella-Infected Chicks. Animals (Basel). 2026. URL: https://pubmed.ncbi.nlm.nih.gov/42071960/ [23] Swaggerty CL, Sasia S, Cabrera MD, et al. Oregano essential oil: a pre-harvest tool to reduce Salmonella enterica serovar Enteritidis in market-age broilers. Poult Sci. 2026. URL: https://pubmed.ncbi.nlm.nih.gov/42061244/ [24] Zhao H, You S, Fu J, et al. Phage therapy: A novel strategy to combat drug-resistant Salmonella Pullorum infection in chickens. Vet Microbiol. 2026. URL: https://pubmed.ncbi.nlm.nih.gov/42061220/ [25] Wei S, Wu J, Zhou L, et al. Isolation of lytic phage and its inhibition of multidrug-resistant Salmonella and its biofilm. Vet Microbiol. 2026. URL: https://pubmed.ncbi.nlm.nih.gov/42000399/ [26] Muntaha ST, Khan GN, Khan I, et al. Isolation, characterization and antibacterial potential of bacteriophages SGP009, SGP004, and SGP007 targeting Salmonella enterica serovar Gallinarum. Int Microbiol. 2026. URL: https://pubmed.ncbi.nlm.nih.gov/41961216/ [27] Li W, Li YA, Liu X, et al. Oral immunization with attenuated Salmonella enterica serovar Enteritidis expressing dual-toxin antigen induces protective immunity against avian [necrotic enteritis](/knowledge/bacteria/avian-bacteria/poultry-necrotic-enteritis-pathogenesis-control). Vaccine. 2026. URL: https://pubmed.ncbi.nlm.nih.gov/42105397/ [28] Zhao Y, Li G, Li YA, et al. Identification and protective efficacy characterization of novel immunogenic antigens for Salmonella Enteritidis vaccines. Poult Sci. 2026. URL: https://pubmed.ncbi.nlm.nih.gov/42105384/ [29] Li W, Li YA, Liu X, et al. Recombinant Attenuated Salmonella Enteritidis Vector Enhances the Immunogenicity of Clostridium perfringens EntB Antigen for Effective Prevention of Avian [Necrotic Enteritis](/knowledge/bacteria/avian-bacteria/necrotic-enteritis-poultry). Biomolecules. 2026. URL: https://pubmed.ncbi.nlm.nih.gov/42072696/ [30] He H, Jiang X, Luo Z, et al. Identification of Salmonella enterica serovar Enteritidis mutants overproducing OMVs as vaccine candidates. Vet Microbiol. 2026. URL: https://pubmed.ncbi.nlm.nih.gov/41955656/ [31] Liu K, Wei S, Liu C, et al. Structural rationality and enhanced immunoprotective efficacy of endogenously expressed OmpX-IL-9 fusion protein against Salmonella in broilers. Protein Expr Purif. 2026. URL: https://pubmed.ncbi.nlm.nih.gov/41966432/