How to Get Certified as a Medical Laboratory Technician (MLT)
By Dr. Zubair Khalid, DVM, MS, PhD ·

Quick Answer
- Complete an accredited associate degree program in medical laboratory technology to qualify for certification eligibility through a recognized certifying agency.
- Pass a national certification examination from an agency such as ASCP BOC or AMT to earn the MLT credential.
- Maintain certification through continuing education credits and renewal fees, with requirements varying by the certifying organization.
Understanding the Medical Laboratory Technician Role
Medical laboratory technicians perform routine diagnostic testing on blood, body fluids, and tissue samples under the supervision of medical laboratory scientists or pathologists. The U.S. Bureau of Labor Statistics groups these professionals with clinical laboratory technologists and technicians, noting that they examine body fluids, cells, and tissue samples for abnormalities and use automated laboratory equipment to conduct tests. Most MLTs work in hospitals, diagnostic laboratories, physician offices, and outpatient care centers, with some positions in blood banks and public health facilities.
The distinction between a medical laboratory technician and a medical laboratory scientist matters for certification planning. An MLT typically completes a two-year associate degree and performs routine testing procedures. A medical laboratory scientist, sometimes called a clinical laboratory scientist, completes a four-year bachelor's degree and takes on more complex testing, method validation, and supervisory responsibilities. The certification pathway you choose depends on which role you intend to fill and the educational level you are willing to complete.
At a Glance: Certification Pathway Overview
| Step | Action | Typical Duration | Key Consideration |
|---|---|---|---|
| Education | Complete NAACLS-accredited MLT associate degree program | 2 years | Accreditation affects exam eligibility |
| Eligibility | Verify your education meets certifying agency requirements | 1 to 2 weeks | Review requirements before applying |
| Application | Submit application and fees to certifying agency | 1 to 4 weeks | Processing times vary by agency |
| Examination | Pass national certification exam | 1 day | Use approved study materials |
| Certification | Receive MLT credential | 2 to 6 weeks after passing | Credential is time-limited |
| Maintenance | Complete continuing education and renewal | Ongoing | Renewal cycles vary by agency |
Education Requirements for MLT Certification
Accredited Degree Programs
The first step toward MLT certification is completing an accredited education program. The National Accrediting Agency for Clinical Laboratory Sciences, known as NAACLS, accredits medical laboratory technician programs across the United States. Graduating from a NAACLS-accredited program is a common eligibility requirement for the major certifying agencies, including the American Society for Clinical Pathology Board of Certification and American Medical Technologists.
MLT programs typically award an associate of applied science degree and include both classroom instruction and clinical rotations. Coursework covers hematology, clinical chemistry, microbiology, immunology, and blood banking. Clinical rotations place you in hospital laboratories or reference laboratories where you practice specimen handling, test performance, and quality control under supervision.
When selecting a program, verify its accreditation status directly with NAACLS. Some programs advertise accreditation that has lapsed or is pending, which can delay your certification application. Contact the program director and ask for the current accreditation letter before enrolling.
Prerequisite Coursework
Most MLT programs require prerequisite courses in biology, chemistry, and mathematics before admission. Typical prerequisites include general biology, general chemistry, and algebra. Some programs also require anatomy and physiology or microbiology. These courses provide the foundational knowledge you need for the technical coursework in the program.
If you are changing careers or have not taken science courses recently, you may need to complete prerequisites at a community college before applying to an MLT program. This adds time to your overall education timeline, so factor it into your planning.
Eligibility Verification for Certification
Before you apply for the certification exam, you must verify that you meet the eligibility requirements of the certifying agency you choose. Each agency publishes its eligibility criteria, and these criteria can change. The two most common certifying agencies for MLTs are the American Society for Clinical Pathology Board of Certification and American Medical Technologists.
ASCP BOC Eligibility
The ASCP Board of Certification offers the MLT certification exam. To be eligible, you must have completed a NAACLS-accredited MLT program within a specific timeframe. The ASCP BOC also requires that you have not failed the exam more than a certain number of times within a defined period. If you have been out of school for several years, you may need to complete additional coursework or refresher training to qualify.
AMT Eligibility
American Medical Technologists offers the Medical Laboratory Technician certification. AMT eligibility also requires graduation from an accredited MLT program. AMT has its own application process and exam schedule, and its requirements may differ slightly from ASCP BOC.
Military and Alternative Pathways
Some certifying agencies offer alternative eligibility pathways for individuals with military laboratory training or equivalent work experience. These pathways are less common and have specific requirements. If you have military training as a laboratory technician, contact the certifying agency directly to determine whether you qualify under an alternative pathway.
Application Process
Once you have verified your eligibility, you submit an application to the certifying agency. The application includes your education records, official transcripts, and a fee. You must also provide proof of identity and may need to submit a background check.
Required Documents
- Official transcripts from your MLT program
- Proof of program accreditation
- Application fee payment
- Government-issued identification
- Any additional documents required by the certifying agency
Application Timeline
The application review process takes time. The certifying agency must verify your education and eligibility before approving you to take the exam. This review can take several weeks, so apply well before your planned exam date. The certifying agency will notify you when your application is approved and provide instructions for scheduling the exam.
Exam Preparation
The certification exam tests your knowledge and skills in the core areas of medical laboratory science. The exam content is based on the competencies taught in accredited MLT programs.
Exam Content Areas
The MLT certification exam covers the following content areas:
- Blood banking and transfusion medicine
- Chemistry
- Hematology
- Immunology
- Microbiology
- Urinalysis and body fluids
- Laboratory operations and safety
Each content area has a specific percentage of questions on the exam. The certifying agency publishes the exam content outline, which lists the topics and their weight. Use this outline to guide your study plan.
Study Materials
The certifying agencies offer study resources, including practice exams and review courses. Professional organizations such as the American Society for Microbiology provide career development resources that can support your preparation. The ASM career services page offers information on professional roles and training that can help you understand the scope of laboratory work.
Study Plan
Create a study plan that covers all exam content areas. Allocate more time to areas where you feel less confident. Use practice exams to identify your weak areas and focus your study there. Study groups with classmates or colleagues can help you stay accountable and learn from others.
Exam Day
The certification exam is administered at a testing center. You will need to bring approved identification and arrive early. The exam is timed, and you will receive your results after the exam. The certifying agency will send you an official score report and, if you pass, your certification.
Taking the Certification Exam
The certification exam is a computer-based test administered at approved testing centers. You will schedule your exam appointment after your application is approved. The exam is timed and you will receive your results on the day of the exam.
Exam Format
The exam consists of multiple-choice questions. The number of questions and the time limit vary by certifying agency. The exam is designed to assess your knowledge and skills in the content areas listed above.
Passing Score
The passing score is set by the certifying agency and is not publicly disclosed. You will receive a pass or fail result. If you fail, you can retake the exam after a waiting period. The certifying agency will provide information on retake policies.
Post-Certification Maintenance
Once you pass the exam, you receive your MLT credential. The credential is time-limited and must be renewed periodically. Renewal requirements vary by certifying agency.
Continuing Education
Most certifying agencies require continuing education credits for renewal. You earn these credits by completing approved educational activities, such as workshops, webinars, and courses. The number of credits required and the renewal cycle vary by agency.
Renewal Fees
Renewal also requires a fee. The fee amount and renewal cycle are set by the certifying agency. You must submit your renewal application and fee before the expiration date to maintain your credential.
Lapsed Certification
If you do not renew your certification, it lapses. A lapsed certification may affect your employment status, as many employers require current certification. If your certification lapses, you may need to retake the exam or complete additional requirements to reinstate it.
Career Pathways and Advancement
The MLT certification is an entry-level credential. With experience and additional education, you can advance to a medical laboratory scientist role. The U.S. Bureau of Labor Statistics notes that medical laboratory technologists typically need a bachelor's degree, while technicians need an associate degree. Advancing to a scientist role requires additional education and possibly additional certification.
Advancement Options
- Complete a bachelor's degree in medical laboratory science
- Pursue certification as a medical laboratory scientist
- Move into a supervisory or management role
- Specialize in a specific laboratory area, such as microbiology or chemistry
Work Settings
MLTs work in a variety of settings. The U.S. Bureau of Labor Statistics identifies hospitals, diagnostic laboratories, physician offices, and outpatient care centers as the primary employers. Some MLTs work in research settings, such as the National Institutes of Health, where laboratory skills are used in research. The NIH Office of Intramural Training and Education provides information on research career development that may be relevant if you are interested in research.
Federal Employment
Federal agencies, including the National Science Foundation, employ laboratory professionals in various roles. The NSF careers page describes scientific and technical work in a federal research organization. Federal employment may have different requirements than private sector employment, so check job postings for specific requirements.
Professional Development
Professional development is important for career growth. The American Society for Microbiology provides career services that include professional development resources, networking opportunities, and training. These resources can help you stay current in the field and advance your career.
Networking
Professional organizations offer networking opportunities through conferences, webinars, and local chapters. Networking can help you learn about job opportunities and connect with other professionals in the field.
Continuing Education
Continuing education is also a requirement for certification renewal but also a way to stay current with new technologies and procedures. Many professional organizations offer continuing education courses and webinars.
Limitations and Considerations
State Licensure
Some states require MLTs to hold a state license in addition to national certification. State licensure requirements vary by state. Check with your state's health department or licensing board to determine if a license is required.
Certification vs. Licensure
Certification is a voluntary credential that demonstrates your knowledge and skills. Licensure is a legal requirement to practice in a state. Some states require both, while others require only one. Understand the difference and the requirements in your state.
Exam Eligibility Changes
Certifying agencies can change their eligibility requirements. Check the certifying agency's website for the most current requirements before applying. Requirements can change without notice, and you do not want to be caught off guard.
Professional Escalation Criteria
If you encounter issues during the certification process, you may need to escalate your concerns. The following situations warrant escalation:
- Your application is rejected and you believe the rejection is in error
- Your exam results are not reported within the expected timeframe
- You have a dispute with the certifying agency regarding your eligibility
- You need to request an accommodation for a disability
In these situations, contact the certifying agency directly. If the issue is not resolved, you may need to contact the agency's customer service or appeals department.
Building a Personal Certification Readiness Portfolio
A certification application is only as strong as the evidence you can produce on demand. Many aspiring MLTs complete an accredited program and then discover that transcripts, clinical rotation logs, or program accreditation letters are missing, outdated, or difficult to obtain months later. A personal certification readiness portfolio solves this problem by organizing every document and record you need before you begin the application process. This portfolio is a working file you maintain from your first day of the MLT program through the day you submit your renewal application.
What to Include in Your Portfolio
Your portfolio should contain the documents that certifying agencies request during application review. The American Society for Clinical Pathology Board of Certification and American Medical Technologists both require official transcripts and proof of program accreditation. The portfolio should hold the following items in a single organized system:
- Official transcripts from every college or university you attended, including prerequisite coursework
- The NAACLS accreditation letter or certificate from your MLT program
- A copy of your program completion certificate or diploma
- Your clinical rotation log showing hours and competencies completed
- A record of any military laboratory training or equivalent experience
- Your government-issued identification information
- A log of all correspondence with the certifying agency, including dates and reference numbers
- Your continuing education credit certificates for renewal cycles
Store physical documents in a dedicated folder and maintain a digital backup. Scan every document at the time you receive it. A cloud storage folder with dated subfolders for each certification stage keeps your records accessible from any device. The U.S. Bureau of Labor Statistics notes that medical laboratory technicians work in hospitals, diagnostic laboratories, and outpatient care centers, and employers in these settings routinely verify certification status. Your portfolio is the tool that makes that verification smooth.
The Portfolio Review Schedule
A portfolio is only useful if it is current. Set a review schedule that aligns with your certification timeline. Review your portfolio at four key points:
- When you enroll in the MLT program
- When you complete your clinical rotations
- When you submit your certification application
- When you renew your certification
At each review, check that every document is present, legible, and current. Verify that your name appears exactly as it does on your government identification. A mismatch between your transcript name and your ID name can delay your application. If you have changed your name, update your records with the certifying agency before you apply.
Tracking Certification Deadlines
Certification deadlines are not optional. Missing a deadline can mean paying a late fee, waiting for the next application cycle, or losing your eligibility window. A simple tracking system prevents this. Use a spreadsheet or a paper calendar with the following columns:
- Task description
- Required action
- Deadline date
- Date completed
- Confirmation number or reference
Enter every deadline from the certifying agency website as soon as you decide to apply. Include the application submission deadline, the document submission deadline, the exam scheduling window, and the exam date. Add reminders at least two weeks before each deadline. This system gives you a clear view of your progress and prevents last-minute scrambling.
Common Portfolio Gaps and How to Fix Them
The most common reason applications are delayed is missing or incomplete documentation. The following gaps appear frequently:
Missing transcripts from prerequisite courses. Many applicants complete prerequisites at a different institution than their MLT program. The certifying agency may require transcripts from every institution you attended. Request these transcripts early, because processing can take several weeks.
Accreditation letter not current. Your program accreditation letter may have an expiration date. If your program's accreditation lapses, you may need to obtain a new letter from the program director. Contact the program director as soon as you know your application timeline.
Name mismatch. Your transcript, your ID, and your application must show the same name. If you have changed your name, provide documentation of the change with your application.
Clinical rotation log incomplete. Some certifying agencies require proof of clinical experience. If your program does not provide a formal log, create your own record of your rotations, including the dates, the facility, and the competencies you performed.
Continuing education records lost. For renewal, you must document your continuing education credits. Keep a running log of every activity you complete, including the provider, the date, and the number of credits. The American Society for Microbiology career services page offers professional development resources that can help you identify approved continuing education activities.
Comparing Certification Agencies Before You Apply
The two most common certifying agencies for MLTs are the American Society for Clinical Pathology Board of Certification and American Medical Technicians. They are not identical. The differences matter for your application timeline, your exam preparation, and your renewal obligations. A side-by-side comparison helps you choose the agency that fits your situation.
Application Requirements Comparison
Both agencies require graduation from a NAACLS-accredited MLT program. The ASCP BOC has a specific eligibility window that requires you to have completed your program within a defined number of years. If you have been out of school for several years, you may need to complete additional coursework or refresher training to qualify. AMT has its own eligibility criteria that may be more flexible for applicants with work experience.
Exam Content and Format
The exam content for both agencies covers the same core areas: blood banking, chemistry, hematology, immunology, microbiology, and urinalysis. The number of questions and the time limit differ between the agencies. The certifying agency publishes the exam content outline, which lists the topics and their weight. Use this outline to guide your study plan.
Renewal Requirements
Renewal cycles and continuing education requirements differ between agencies. One agency may require a certain number of continuing education credits every three years, while the other requires a different number on a different cycle. The renewal fee also differs. Check the current requirements on the agency website before you commit to one agency.
How to Make the Choice
Your choice of certifying agency should be based on your employment plans and your personal circumstances. If your employer requires a specific certification, choose that agency. If you plan to move to a state with specific licensure requirements, check whether that state recognizes one agency over the other. If you have no preference, choose the agency whose renewal cycle and fee structure fit your budget and schedule.
A Practical Record-Keeping Method for Continuing Education
Continuing education is a requirement for certification renewal, but it is also a professional development opportunity. The American Society for Microbiology provides career services that include professional development resources and training. The National Institutes of Health Office of Intramural Training and Education also offers training and professional development context that can help you build your skills.
The Continuing Education Log
Create a simple log with the following columns:
- Activity date
- Activity title
- Provider name
- Number of credits
- Certificate or proof of completion
- Date submitted for renewal
Update this log every time you complete an activity. Do not wait until renewal time to reconstruct your records. A current log makes renewal straightforward and protects you if the certifying agency audits your credits.
Choosing Approved Activities
Not every educational activity counts toward your renewal. The certifying agency approves specific providers and activity types. Before you pay for a course or webinar, verify that the provider is approved by your certifying agency. The agency website lists approved providers and activity categories. When in doubt, contact the agency directly.
The Audit Risk
Certifying agencies may audit a percentage of renewal applications. An audit requires you to submit proof of your continuing education credits. If you cannot produce the certificates or logs, your renewal may be delayed or denied. A complete log with attached certificates protects you in an audit.
When to Escalate a Certification Problem
Most certification issues are resolved by contacting the certifying agency directly. Some situations require escalation. The following criteria indicate that you should escalate your concern:
- Your application has been rejected and you believe the rejection is in error
- Your exam results have not been reported within the expected timeframe
- You have a dispute with the certifying agency regarding your eligibility
- You need to request an accommodation for a disability
- Your renewal application has been denied and you believe the denial is in error
In these situations, contact the certifying agency's customer service department first. If the issue is not resolved, ask for the appeals department. Document every interaction, including the date, the representative's name, and the outcome. This documentation is your evidence if you need to escalate further.
Records You Must Keep After Certification
Your record-keeping does not end when you pass the exam. You must maintain records for the entire period you hold the credential. The following records are essential:
- Your certification certificate and number
- Your renewal confirmation notices
- Your continuing education logs and certificates
- Your correspondence with the certifying agency
- Your state licensure documents, if applicable
Keep these records in your portfolio and update them at each renewal cycle. The U.S. Bureau of Labor Statistics notes that medical laboratory technicians are employed in a variety of settings, and employers may verify your certification status at any time. A complete record system makes that verification simple and protects your employment status.
Frequently Asked Questions
What is the difference between an MLT and a medical laboratory scientist?
An MLT typically holds an associate degree and performs routine testing procedures. A medical laboratory scientist holds a bachelor's degree and performs more complex testing, method validation, and supervisory duties. The U.S. Bureau of Labor Statistics distinguishes between the two roles based on education level and job duties.
How long does it take to become a certified MLT?
The typical path takes about two years for the associate degree program, plus additional time for prerequisites and the certification exam. The total time depends on your starting point and whether you need to complete prerequisite courses.
Can I get certified without a degree?
Most certifying agencies require graduation from an accredited MLT program. Some agencies offer alternative pathways for military training or work experience, but these are limited. Check with the certifying agency for specific requirements.
How much does the certification exam cost?
The exam fee varies by certifying agency. The fee is set by the agency and can change. Check the certifying agency website for the current fee schedule.
How often do I need to renew my certification?
Renewal cycles vary by certifying agency. Most require renewal every few years, with continuing education credits and a renewal fee. Check with your certifying agency for the specific renewal requirements.
What happens if I fail the certification exam?
If you fail the exam, you can retake it after a waiting period. The certifying agency will provide information on retaking procedures and any additional requirements.
Do I need a state license in addition to certification?
Some states require a state license for MLTs. State requirements vary. Check with your state's health department or licensing board to determine if a license is required.
Can I work in a research setting with an MLT certification?
Yes, MLTs can work in research settings. The National Institutes of Health and other research organizations employ laboratory professionals. The NIH Office of Intramural Training and Education provides information on research career development.
Using the Evidence
| Source | Best use in this topic | Important limitation |
|---|---|---|
| Occupational Outlook Handbook | official guidance | Check the linked page for current local requirements |
| O*NET OnLine | official guidance | Check the linked page for current local requirements |
| Office of Intramural Training and Education | official guidance | Check the linked page for current local requirements |
Related Articles
- Medical Laboratory Career Paths: From Technician to Director
- Medical Laboratory Careers: A Practical Guide to Roles, Education, and Advancement
- Medical Assistant Certification Programs
- Clinical Laboratory Careers: Certification and Advancement
- Laboratory Medicine Careers: Roles, Education, and Opportunities
References and Further Reading
- Occupational Outlook Handbook. U.S. Bureau of Labor Statistics.
- O*NET OnLine. U.S. Department of Labor.
- Office of Intramural Training and Education. National Institutes of Health.
- Careers at NSF. U.S. National Science Foundation.
- Career Services. American Society for Microbiology.
- AAVMC Career Resources. Association of American Veterinary Medical Colleges.
- NCBI Literature Resources. National Center for Biotechnology Information.
- PubMed. National Library of Medicine.
This article is educational and does not replace institutional policy, professional advice, or applicable safety and regulatory requirements.